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KMID : 0613820060160020282
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Journal of Life Science
2006 Volume.16 No. 2 p.282 ~ p.288
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Antitumor Sterol Isolated from the Fruiting Body of Pleurotus eryngii
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Lee Young-Hoon
Park Ki-Hoon
Lee Byong-Won
Cho Yong-Un
Choi Young-Ju
Gal Sang-Wan
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Abstract
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Activity-guided fractionations led to the isolation of antitumor compound, ergosterol peroxide (5α, 8α-epideoxy-24(R)-methylcholesta-6, 22-dien-3β-ol) from the fruiting body of Pleurotus eryngii that was cultivated artificially. This sterol structure was established by using spectroscopic methods (1H and ¹³C nuclear magnetic resonance and high resolution mass spectra). The purified compound showed a molecular formular of C28H₄₄O₃, displaying characteristic features of epidioxy sterols. The 50% inhibitory concentrations (IC50) of ergosterol peroxide against human lung cancer cell line (A549) and human ovarian cell line (SK-OV3) were 7 μM and 14 μM, respectively. In the DNA fragmentation assay, the compound showed the programmed cell death causing the chromosomal DNA fragmentation. It reveals that ergosterol peroxide arrests G1 phase of the cell division cycle.
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KEYWORD
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Pleurotus eryngii, ergosterol peroxide, 50% inhibitory concentration, A549, SK-OV3
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